stainless steel screens (2-µm pores) Search Results


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Sterlitech corporation silver membrane filter
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FUJIFILM purmorphamine
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Xcessbio Inc thz1
(A) Left panel: Schematic of the genomic locus of CSB and used strategy for generating the homozygous CSB-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for CSB -KI cell line. (B) Left panel: Schematic of the genomic locus of UVSSA and used strategy for generating the homozygous UVSSA-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for UVSSA -KI cell line. (C) Left panel: CSB mobility was determined by FRAP analysis of CSB-mScarletI after the indicated treatments. <t>THZ1:</t> 1 hour treatment (2 µM) before UV-C irradiation (4 J/m 2 ) or mock treatment. Right panel: Relative immobile fraction of CSB as determined by FRAP analysis. Plotted values represent mean ± SEM and are normalized to mock treated. n≥15 cells. (D) Same as C but for UVSSA-mScarletI. n≥10 cells. (E+F) FRAP analyses of CSB-mScarletI (E) or UVSSA-mScarletI (F) mobility after transfection with indicated siRNAs in individual graphs. Cells were mock treated (NT) or analyzed directly (UV) or 5 hours (5hr UV) after irradiation with 4 J/m 2 UV-C. (G) Relative fluorescence intensity of UVSSA in UVSSA -KI cells transfected with indicated siRNAs as determined by live-cell imaging. Plotted values represent mean ± SEM. n≥16 cells. (H) FRAP analysis of CSB in CSB -KI cells transfected with indicated siRNAs 2 hours after UV. VCPi: VCP inhibitor (5 µM) was directly added after UV-C (4 J/m 2 ). (I) Immunoblot of chromatin fraction of indicated cell lines 1 hour after 12 J/m 2 UV-C or mock treatment. NAEi = 1 hour treatment with NEDDylation inhibitor (10 µM). SSRP1 is shown as loading control. (J) Immunoblot of chromatin fraction of HCT116 cells transfected with indicated siRNAs 1 hour after 12 J/m 2 UV-C or mock treatment. SSRP1 is shown as loading control.
Thz1, supplied by Xcessbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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QImaging ccd camera
(A) Left panel: Schematic of the genomic locus of CSB and used strategy for generating the homozygous CSB-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for CSB -KI cell line. (B) Left panel: Schematic of the genomic locus of UVSSA and used strategy for generating the homozygous UVSSA-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for UVSSA -KI cell line. (C) Left panel: CSB mobility was determined by FRAP analysis of CSB-mScarletI after the indicated treatments. <t>THZ1:</t> 1 hour treatment (2 µM) before UV-C irradiation (4 J/m 2 ) or mock treatment. Right panel: Relative immobile fraction of CSB as determined by FRAP analysis. Plotted values represent mean ± SEM and are normalized to mock treated. n≥15 cells. (D) Same as C but for UVSSA-mScarletI. n≥10 cells. (E+F) FRAP analyses of CSB-mScarletI (E) or UVSSA-mScarletI (F) mobility after transfection with indicated siRNAs in individual graphs. Cells were mock treated (NT) or analyzed directly (UV) or 5 hours (5hr UV) after irradiation with 4 J/m 2 UV-C. (G) Relative fluorescence intensity of UVSSA in UVSSA -KI cells transfected with indicated siRNAs as determined by live-cell imaging. Plotted values represent mean ± SEM. n≥16 cells. (H) FRAP analysis of CSB in CSB -KI cells transfected with indicated siRNAs 2 hours after UV. VCPi: VCP inhibitor (5 µM) was directly added after UV-C (4 J/m 2 ). (I) Immunoblot of chromatin fraction of indicated cell lines 1 hour after 12 J/m 2 UV-C or mock treatment. NAEi = 1 hour treatment with NEDDylation inhibitor (10 µM). SSRP1 is shown as loading control. (J) Immunoblot of chromatin fraction of HCT116 cells transfected with indicated siRNAs 1 hour after 12 J/m 2 UV-C or mock treatment. SSRP1 is shown as loading control.
Ccd Camera, supplied by QImaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stainless+steel+screens+(2-%C2%B5m+pores)/ccd+camera/pmc03252314-50-9-12
Average 90 stars, based on 1 article reviews
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90
Becton Dickinson golgistop
(A) Left panel: Schematic of the genomic locus of CSB and used strategy for generating the homozygous CSB-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for CSB -KI cell line. (B) Left panel: Schematic of the genomic locus of UVSSA and used strategy for generating the homozygous UVSSA-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for UVSSA -KI cell line. (C) Left panel: CSB mobility was determined by FRAP analysis of CSB-mScarletI after the indicated treatments. <t>THZ1:</t> 1 hour treatment (2 µM) before UV-C irradiation (4 J/m 2 ) or mock treatment. Right panel: Relative immobile fraction of CSB as determined by FRAP analysis. Plotted values represent mean ± SEM and are normalized to mock treated. n≥15 cells. (D) Same as C but for UVSSA-mScarletI. n≥10 cells. (E+F) FRAP analyses of CSB-mScarletI (E) or UVSSA-mScarletI (F) mobility after transfection with indicated siRNAs in individual graphs. Cells were mock treated (NT) or analyzed directly (UV) or 5 hours (5hr UV) after irradiation with 4 J/m 2 UV-C. (G) Relative fluorescence intensity of UVSSA in UVSSA -KI cells transfected with indicated siRNAs as determined by live-cell imaging. Plotted values represent mean ± SEM. n≥16 cells. (H) FRAP analysis of CSB in CSB -KI cells transfected with indicated siRNAs 2 hours after UV. VCPi: VCP inhibitor (5 µM) was directly added after UV-C (4 J/m 2 ). (I) Immunoblot of chromatin fraction of indicated cell lines 1 hour after 12 J/m 2 UV-C or mock treatment. NAEi = 1 hour treatment with NEDDylation inhibitor (10 µM). SSRP1 is shown as loading control. (J) Immunoblot of chromatin fraction of HCT116 cells transfected with indicated siRNAs 1 hour after 12 J/m 2 UV-C or mock treatment. SSRP1 is shown as loading control.
Golgistop, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BIA Separations cim r-protein g lld monolithic 96-well plate ( channels)
(A) Left panel: Schematic of the genomic locus of CSB and used strategy for generating the homozygous CSB-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for CSB -KI cell line. (B) Left panel: Schematic of the genomic locus of UVSSA and used strategy for generating the homozygous UVSSA-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for UVSSA -KI cell line. (C) Left panel: CSB mobility was determined by FRAP analysis of CSB-mScarletI after the indicated treatments. <t>THZ1:</t> 1 hour treatment (2 µM) before UV-C irradiation (4 J/m 2 ) or mock treatment. Right panel: Relative immobile fraction of CSB as determined by FRAP analysis. Plotted values represent mean ± SEM and are normalized to mock treated. n≥15 cells. (D) Same as C but for UVSSA-mScarletI. n≥10 cells. (E+F) FRAP analyses of CSB-mScarletI (E) or UVSSA-mScarletI (F) mobility after transfection with indicated siRNAs in individual graphs. Cells were mock treated (NT) or analyzed directly (UV) or 5 hours (5hr UV) after irradiation with 4 J/m 2 UV-C. (G) Relative fluorescence intensity of UVSSA in UVSSA -KI cells transfected with indicated siRNAs as determined by live-cell imaging. Plotted values represent mean ± SEM. n≥16 cells. (H) FRAP analysis of CSB in CSB -KI cells transfected with indicated siRNAs 2 hours after UV. VCPi: VCP inhibitor (5 µM) was directly added after UV-C (4 J/m 2 ). (I) Immunoblot of chromatin fraction of indicated cell lines 1 hour after 12 J/m 2 UV-C or mock treatment. NAEi = 1 hour treatment with NEDDylation inhibitor (10 µM). SSRP1 is shown as loading control. (J) Immunoblot of chromatin fraction of HCT116 cells transfected with indicated siRNAs 1 hour after 12 J/m 2 UV-C or mock treatment. SSRP1 is shown as loading control.
Cim R Protein G Lld Monolithic 96 Well Plate ( Channels), supplied by BIA Separations, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Merck KGaA purmorphamine
(A) Left panel: Schematic of the genomic locus of CSB and used strategy for generating the homozygous CSB-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for CSB -KI cell line. (B) Left panel: Schematic of the genomic locus of UVSSA and used strategy for generating the homozygous UVSSA-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for UVSSA -KI cell line. (C) Left panel: CSB mobility was determined by FRAP analysis of CSB-mScarletI after the indicated treatments. <t>THZ1:</t> 1 hour treatment (2 µM) before UV-C irradiation (4 J/m 2 ) or mock treatment. Right panel: Relative immobile fraction of CSB as determined by FRAP analysis. Plotted values represent mean ± SEM and are normalized to mock treated. n≥15 cells. (D) Same as C but for UVSSA-mScarletI. n≥10 cells. (E+F) FRAP analyses of CSB-mScarletI (E) or UVSSA-mScarletI (F) mobility after transfection with indicated siRNAs in individual graphs. Cells were mock treated (NT) or analyzed directly (UV) or 5 hours (5hr UV) after irradiation with 4 J/m 2 UV-C. (G) Relative fluorescence intensity of UVSSA in UVSSA -KI cells transfected with indicated siRNAs as determined by live-cell imaging. Plotted values represent mean ± SEM. n≥16 cells. (H) FRAP analysis of CSB in CSB -KI cells transfected with indicated siRNAs 2 hours after UV. VCPi: VCP inhibitor (5 µM) was directly added after UV-C (4 J/m 2 ). (I) Immunoblot of chromatin fraction of indicated cell lines 1 hour after 12 J/m 2 UV-C or mock treatment. NAEi = 1 hour treatment with NEDDylation inhibitor (10 µM). SSRP1 is shown as loading control. (J) Immunoblot of chromatin fraction of HCT116 cells transfected with indicated siRNAs 1 hour after 12 J/m 2 UV-C or mock treatment. SSRP1 is shown as loading control.
Purmorphamine, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Left panel: Schematic of the genomic locus of CSB and used strategy for generating the homozygous CSB-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for CSB -KI cell line. (B) Left panel: Schematic of the genomic locus of UVSSA and used strategy for generating the homozygous UVSSA-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for UVSSA -KI cell line. (C) Left panel: CSB mobility was determined by FRAP analysis of CSB-mScarletI after the indicated treatments. THZ1: 1 hour treatment (2 µM) before UV-C irradiation (4 J/m 2 ) or mock treatment. Right panel: Relative immobile fraction of CSB as determined by FRAP analysis. Plotted values represent mean ± SEM and are normalized to mock treated. n≥15 cells. (D) Same as C but for UVSSA-mScarletI. n≥10 cells. (E+F) FRAP analyses of CSB-mScarletI (E) or UVSSA-mScarletI (F) mobility after transfection with indicated siRNAs in individual graphs. Cells were mock treated (NT) or analyzed directly (UV) or 5 hours (5hr UV) after irradiation with 4 J/m 2 UV-C. (G) Relative fluorescence intensity of UVSSA in UVSSA -KI cells transfected with indicated siRNAs as determined by live-cell imaging. Plotted values represent mean ± SEM. n≥16 cells. (H) FRAP analysis of CSB in CSB -KI cells transfected with indicated siRNAs 2 hours after UV. VCPi: VCP inhibitor (5 µM) was directly added after UV-C (4 J/m 2 ). (I) Immunoblot of chromatin fraction of indicated cell lines 1 hour after 12 J/m 2 UV-C or mock treatment. NAEi = 1 hour treatment with NEDDylation inhibitor (10 µM). SSRP1 is shown as loading control. (J) Immunoblot of chromatin fraction of HCT116 cells transfected with indicated siRNAs 1 hour after 12 J/m 2 UV-C or mock treatment. SSRP1 is shown as loading control.

Journal: bioRxiv

Article Title: Elongation factor ELOF1 drives transcription-coupled repair and prevents genome instability

doi: 10.1101/2021.05.11.443558

Figure Lengend Snippet: (A) Left panel: Schematic of the genomic locus of CSB and used strategy for generating the homozygous CSB-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for CSB -KI cell line. (B) Left panel: Schematic of the genomic locus of UVSSA and used strategy for generating the homozygous UVSSA-mScarletI-HA KI cell line. Half arrows indicate primer locations. Middle and right panel: Genotyping PCR and immunoblot for UVSSA -KI cell line. (C) Left panel: CSB mobility was determined by FRAP analysis of CSB-mScarletI after the indicated treatments. THZ1: 1 hour treatment (2 µM) before UV-C irradiation (4 J/m 2 ) or mock treatment. Right panel: Relative immobile fraction of CSB as determined by FRAP analysis. Plotted values represent mean ± SEM and are normalized to mock treated. n≥15 cells. (D) Same as C but for UVSSA-mScarletI. n≥10 cells. (E+F) FRAP analyses of CSB-mScarletI (E) or UVSSA-mScarletI (F) mobility after transfection with indicated siRNAs in individual graphs. Cells were mock treated (NT) or analyzed directly (UV) or 5 hours (5hr UV) after irradiation with 4 J/m 2 UV-C. (G) Relative fluorescence intensity of UVSSA in UVSSA -KI cells transfected with indicated siRNAs as determined by live-cell imaging. Plotted values represent mean ± SEM. n≥16 cells. (H) FRAP analysis of CSB in CSB -KI cells transfected with indicated siRNAs 2 hours after UV. VCPi: VCP inhibitor (5 µM) was directly added after UV-C (4 J/m 2 ). (I) Immunoblot of chromatin fraction of indicated cell lines 1 hour after 12 J/m 2 UV-C or mock treatment. NAEi = 1 hour treatment with NEDDylation inhibitor (10 µM). SSRP1 is shown as loading control. (J) Immunoblot of chromatin fraction of HCT116 cells transfected with indicated siRNAs 1 hour after 12 J/m 2 UV-C or mock treatment. SSRP1 is shown as loading control.

Article Snippet: Cell were treated for 1 hour with the following chemicals: Actinomycin D (Sigma, 1 µg/ml), Flavopiridol (Sigma, 1 µM), THZ1 (Xcessbio, 2 µM), Mitomycin C (Sigma, 10 µg/ml unless indicated differently), or potassium bromate (KBrO 3 , Sigma).

Techniques: Western Blot, Irradiation, Transfection, Fluorescence, Live Cell Imaging